Introduction & Overview

Mass spectrometry (MS) is a powerful analytical technique that is used to determine the molecular mass of molecules by measuring the mass-to-charge (m/z) ratio of respective ions. The MS principle consists of ionizing neutral molecules to generate charged molecules or fragment ions, which are further separated according to their m/z values and finally detected. The application of mass spectrometry to drugs, synthetic organic molecules, active pharmaceutical ingredients (APIs), natural products, peptides, proteins, carbohydrates, nucleic acids, etc. is well known.

The hyphenation of liquid chromatography (LC), a photodiode-array UV-Vis detector, and atmospheric pressure ionization mass spectrometry (APIMS) have proven to be an efficient online system for establishing the identity of organic compounds in any given test sample. A broad range of molecules, such as volatile, non-volatile, ionic, etc., can be analyzed at the expense of a very small sample. A high-resolution mass spectrometer with tandem mass spectrometric (MS/MS) analysis is proficient in identifying unknown compounds, quantifying known compounds, and elucidating the structure of molecules. The mass spectrometry facility at CDRI consists of state-of-the-art mass spectrometers.

Capabilities, expertise and services offered

Small molecules analysis: https://www.saiflucknow.org

• ESI/APCI-MS analysis.
• Qualitative LC/MS method development and analysis.
• Quantitative LC/MS method development, validation and analysis.
• High resolution mass spectral analysis (HRMS).

Trainings:

We offer training programs to motivate interested postgraduate students and research fellows with an opportunity to spend valuable time gaining practical experience in the field of mass spectrometry. We offer different time-bound training programs: The course fee may vary based on duration and technique. https://www.saiflucknow.org/traning and https://cdri.res.in/skilldevelopmentpostgraduate.aspx

 Workshops:

Awareness workshops are being held every year in CSIR-CDRI, Lucknow, to keep users informed about the scientific advancements in these areas. This program also provides a platform for the younger generation to explore the application of sophisticated instruments for their research work. https://www.saiflucknow.org/traning

 Sponsored projects:

We take up sponsored projects to analyze small molecules present in complex matrices, qualitative and quantitative analysis of targeted compounds (pharmaceuticals), and the generation of HPLC/PDA and mass spectrum fingerprints of herbal products. Standardization of herbal medicines (HMs), LC/MS-based phytochemical analysis (dereplication), quantification of chemical markers, quality testing, and authentication of raw plant materials using mass spectrum fingerprints (MSFP) library (https://plantmetabolome.cdri.res.in).

 Open access facility:

TMS database (https://tmsdatabase.cdri.res.in) is a library of MS/MS spectrum of naturally occurring compounds (Natural products). Digital library of Indian medicinal plants and their metabolites (https://plantmetabolome.cdri.res.in).

Major Equipment and Instrumentation

1. Waters Xevo TQD Triple Quadrupole Mass Spectrometer Hyphenated with a Waters Acquity H-Class UPLC/PDA system Ionization Mode : ESI and ESCi Mass Range : m/z 50-2000 Th Mass Resolution : > Unit Mass (Da), Full scan MS and MS/MS, SIR and MRM Qualitative LC/MS method development and analysis.
2. Waters TQD Triple Quadrupole Mass Spectrometer Hyphenated with a Waters Alliance e2695 Separations/2424 ELSD system Ionization Mode : ESI and ESCi Mass Range : m/z 50-2000 Th Mass Resolution : Unit Mass (Da), Full scan MS and MS/MS, SIR and MRM Qualitative LC/MS method development and analysis.
3. AB Sciex 4000 Q-Trap-Mass Spectrometer, Hyphenated with a Waters Acquity UPLC system Ionization Mode : ESI Mass Range : m/z 30-2800 (quadrupole mode) and m/z 50-2800 Linear Ion Trap mode. Mass Resolution : >3000 at m/z 609 at 250 amu/sec Quantitative LC/MS method development, validation and analysis.
4. Waters Alliance e2695 Separations with 2998 Photodiode Array (PDA) Detector Wavelength Range : 190-800 nm Wavelength Accuracy : ±1 nm Linear Dynamic Range : 4.5 orders Flow Rate : 0.1 to 10 ml/min Qualitative and quantitative HPLC method development, validation and analysis.

Sample Analysis

Charges & Sample requirement

Application Form

Online Application: https://www.saiflucknow.org

Faculty & Technical Personnel

• Dr. Sanjeev Kanojiya
  Facility-in-Charge MS Unit
  Senior Principal Scientist & Professor (AcSIR)

Technical Personnel:

• 1. Mr. Dharmesh kumar, Sr. Technical Officer
• 2. Dr. Tofan Kumar, Sr. Technical Officer
• 3. Ms. Pooja Soni, Sr. Technical Officer
• 4. Dr. Vipin Kumar, Technical Assistant
• 5. Ms. Pooja Singh, Technical Assistant
• 6. Mr. S. A. Singh, Sr. Technician

Research Fellows:

• 1. Dr. Trapti Joshi (UGC-JRF), 2012-2018
• 2. Dr. Yatendra (CSIR-JRF), 2017-2022
• 3. Dr. Priyanka Rawat (UGC-JRF), 2018-2023
• 4. Dr. Pratibha Singh (ICMR-SRF), 2019-2023
• 5. Dr. Vijaya Shukla (ICMR-SRF), 2019-2023
• 6. Mr. Ashish Singh (UGC-JRF), 2019
• 7. Mr. Akhilesh Kumar (UGC-JRF), 2022
• 8. Mr. Arvind Kumar Maurya (UGC-JRF), 2022
• 9. Mr. Mohsin Ali (CSIR-JRF), 2023
• 10. Mr. Ashish Singh Tanwar (DST-Inspire JRF), 2023

Selected Publications:

1. 1. Rawat P, Sharma K, Khanka S, Singh Y, Prakash R, Rais N, Maurya AK, Gupta R, Mishra DK, Singh D,Kanojiya S. (2024) Bone Fracture-healing Properties and UPLC-MS Analysis of an Enriched Flavonoid Fraction from Oxystelma esculentum. Planta Med, 90, 96-110.


2. 2. Singh Y, Rawat P, Kumar A, Singh SK, Mishra DK,Kanojiya S. (2023) Exploration of new and alternative sources of targeted bioflavonoids using ultra-performance liquid chromatography-tandem mass spectrometry. SEPARATION SCIENCE PLUS, 6, 2300020.


3. 3. Rawat P, Singh Y, Tiwari S, Mishra DK,Kanojiya S. (2023) Structures characterization and quantification of Cajanus scarabaeoides phytochemicals and their seasonal variation analysis utilizing ultra-performance liquid chromatography-tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 15; 37(3), e9440.


4. 4. Singh Y, Nimoriya R, Rawat P, Mishra DK, Kanojiya S. (2022) Quantitative evaluation of cardiac glycosides and their seasonal variation analysis in Nerium oleander using UHPLC-ESI-MS/MS. Phytochem Anal. 33(5):746-753.


5. 5. Singh Y, Nimoriya R, Rawat P, Mishra DK,Kanojiya S. (2021) Structural Analysis of Diastereomeric Cardiac Glycosides and Their Genins Using Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 32, 1205-1214.