Octet R8 (BLI - Bio-Layer Interferometry) simultaneously collect data from up to eight “dip-and-read” sensors. This instrument uses 96-well plates for sample handling. The molecule of interest ("ligand") is immobilized on the surface of a sensor, creating the biolayer. When this sensor is dipped into a well containing a solution of the ligand’s binding partner ("analyte"), the analyte molecules bind to the immobilized ligand molecules, changing the biolayer’s thickness. To monitor the biolayer thickness changes, Octet analyzes the interference of the light beam reflected from the tip of the sensor. In this way, Octet can track the association and dissociation of the analyte from the immobilized ligand in real time. It can use crude sample solutions, such as cell culture supernatants, diluted serum, etc. The BLI signal is sensitive to the binding of a large range of analytes, from small molecules (MW > 150 Da) to virus particles. Most sensors can be regenerated and used multiple times.