Main Areas of Activity

The EM Unit houses three microscopy facilities –

(i) 120 kV JEOL Transmission Electron Microscope,
(ii) FEI Quanta 250 Scanning Electron Microscope and
(iii) Carl Zeiss LSM510 META Laser Scanning Confocal Microscope.

Annually, these facilities are used by more than 40 scientists and their students within the Institute. The EM Unit participates and contributes significantly to all disease areas of the institute by using its expertise in electron and confocal microscopy to carry out ultra-structural studies and sub-cellular localization of macromolecules of biological interest. We also carry out morphological characterization of drug delivery systems and biological nanostructures and are also involved in the development of novel fluorescent probes for cell imaging applications. EM experiments are designed, executed and interpreted in this laboratory. The scientists of the unit have been involved in teaching JNU/AcSIR pre-Ph.D. coursework, in external and internal doctoral committees, in training and also in organizing workshops in the areas of EM and confocal microscopy. Scientists of the unit are regularly invited to deliver lectures at national conferences and workshops and on several occasions, have been invited to assist other institutes within and outside CSIR to plan and successfully set up their EM and confocal microscopy facilities.
The facilities are also open to the external scientific community for availing services provided on payment basis.

Major Equipment and Instrumentation

Transmission Electron Microscope

Model: Jeol JEM 1400 (Jeol Ltd., Tokyo, Japan)



• Easily changeable accelerating voltages from 40-120 KeV
• Tungsten emitter; motorized goniometer
• Magnification up to 300000X
• Equipped with Gatan Erlangshen and Orius CCD cameras
• Anti-contaminator device



High resolution /high contrast lens system; Minimum/low dose imaging for radiation sensitive samples; option for LaB6 emitter; tomography



Resolution: Point: 0.38 nm/lattice: 0.2 nm



Nano-characterization; Negative staining TEM; ultra-structural studies of biological specimens




Electron Microscopy Unit


Scanning Electron Microscope

Model: FEI Quanta 250 (FEI, The Netherlands)



• High Tension 200V – 30 kV; TMP pumped system
• Tungsten emitter
• 2 inch/50 mm 4-axis motorized stage equipped with through-the-lens differential pumping
• Detectors: SE; BSED and LFD
• High vacuum (HV) and low vacuum (LV) modes
• Magnification up to 1000000X



Multi-stub holder can hold upto 7 specimens; Provision for observation of samples with minimum sample prep.



Resolution: 3 nm at 30 kV with SE and 4 nm with BSE detector (HV mode)



Surface morphology analysis of all kinds of conductive and non-conductive samples.




Electron Microscopy Unit

Confocal Laser Scanning Microscope

Model: Carl Zeiss LSM 510 META (Carl Zeiss, Germany)



• LSM 510 META with Axio Observer.Z1 inverted microscope with the following objectives – Plan Apo 10X/0.45; Plan Apo 20X/0.8; Plan Apo 40X/0.95 Korr; Plan Apo 63X/1.4 Oil DIC; Plan Apo 63X/1.4 Oil Ph; Plan Apo 100X/1.4 Oil DIC
• 2 photomultiplier tubes, 1 Meta detector and 1 Transmitted Light detector
• LSM / AIM software for imaging and analysis



Multi-colour localization of fluorescent signals; Z-sectioning and 3D reconstruction; Co-localization; Emission fingerprinting; Spectral un-mixing



High resolution multi-colour localization of fluorescent signals; optical sectioning; 2D/3D confocal imaging



Electron Microscopy Unit

Faculty & Technical Personnel

•Dr. Kalyan Mitra, Senior Scientist and In-charge EM Unit
•Dr. (Mrs) Kavita Singh, Technical Officer
• Ms. Garima Pant, Technical Assistant
•Mrs. Madhuli Srivastava, Senior Technician

Research Fellows

•Mr. Manoj Kathuria, CSIR-SRF
•Mr. Arindam Bhattacharjee, CSIR-SRF
• Ms. Bhanupriya Awasthi, ICMR-SRF
•Mr. Rohit Sahai, DBT-JRF

Services

We also offer the following services to external users on payment basis:

Services offered for TEM/ SEM/confocal

Charges (Rs.) Private industries/Others
TEM (Model: Jeol JEM1400 /120 kV)
Negative staining (per sample)
Viewing grids / imaging (per hr)

1000/500
3000/2000
SEM (Model: FEI Quanta 250)
Specimen Preparation per sample
Viewing per hr

1500
2500/1500
Confocal microscopy (Model: Carl Zeiss LSM 510 META)
Imaging/Viewing prepared slides (per hr)
3000/2000
Custom carbon coated gridsRs. 200/- per grid


• 14.5% service tax extra charged.
• Service charges are payable in advance by crossed bank draft in favour of the Director, Central Drug Research Institute, Lucknow-226031.
• Prior booking/appointment has to be confirmed by writing to Dr. K. Mitra (In-charge EM Unit) at k_mitra@cdri.res.in.

Recent Selected Publications
image01 Hasanain M, Bhattacharjee A, Pandey P, Ashraf R, Singh N, Sharma S, Vishwakarma AL, Datta D, Mitra K*, Sarkar J*. α-Solanine induces ROS-mediated autophagy through activation of endoplasmic reticulum stress and inhibition of Akt/mTOR pathway. Cell Death Dis. 2015 Aug 27;6:e1860. doi:10.1038/cddis.2015.219. PubMed PMID: 26313911.
image01 Bhaskar, Mitra K, Kuldeep J, Siddiqi MI, Goyal N. The TCP1γ subunit of Leishmania donovani forms a biologically active homo-oligomeric complex. FEBS J. 2015 Dec;282(23):4607-19. doi: 10.1111/febs.13521. Epub 2015 Oct 26. PubMed PMID:26395202. 
image01Kathuria M, Bhattacharjee A, Sashidhara KV, Singh SP, Mitra K*. Induction of mitochondrial dysfunction and oxidative stress in Leishmania donovani by orally active clerodane diterpene. Antimicrob Agents Chemother. 2014 Oct;58(10):5916-28. doi: 10.1128/AAC.02459-14. Epub 2014 Jul 28. PubMed PMID: 25070112; PubMed Central PMCID: PMC4187897 
image01 4) Goel A*, Sharma A, Kathuria M, Bhattacharjee A, Verma A, Mishra PR, Nazir A, Mitra K*. New fluoranthene FLUN-550 as a fluorescent probe for selective staining and quantification of intracellular lipid droplets. Org Lett. 2014 Feb 7;16(3):756-9. doi: 10.1021/ol403470d. Epub 2014 Jan 10. PubMed PMID: 24410145.