Model Assay system Disease
FRET assays for screening of small anti-cancer molecules   Assay   Anti -cancer
MTT assay Assay Anti -cancer
Sulphorhodamine B assay (SRB) Assay Anti -cancer  
Trypan Blue exclusion   Assay   Anti -cancer  
BRDU incorporation assay Assay Anti -cancer
Anexin V/PI assay Assay   Anti -cancer  
Real-time PCR-based methods for specific apoptotic/survival gene expressions Assay   Anti -cancer  
Western based methods for specific apoptotic/survival gene expressions   Assay   Anti-Cancer  
Trypan Blue exclusion Assay   Anti -cancer  
Anexin V/PI assay Assay   Anti -cancer  
pTK-(C/EBP)-Luc assay   Assay   Anti -cancer  
Real-time PCR-based methods for specific apoptotic/survival gene expressions   Assay   Anti -cancer  
Western based methods for specific apoptotic/survival gene expressions   Assay   Anti -cancer  
Cytospin based Giemsa staining for assessment of differentiation   Assay   Anti -cancer  
FACS based assay for analysis of differentiation and Apoptosis   Assay   Anti -cancer  
Gelshift Assay   Assay   Anti -cancer  
In-vivo ubiquitination Assay   Assay   Anti -cancer  
Crystal violet Assay for assessment of cell growth   Assay   Anti -cancer  
Patient derived primary cultures for drug screening   Assay   Anti -cancer  
In vitro cell based assays for screening of nuclear receptors  Assay   Anti -cancer  
 standardized.Proteomic screening of anticancer agents Assay   Anti -cancer 
 Wound healing assay In vitro  Cancer 
Migration and invasion assays In vitro   Cancer
 DMBA-induced mammary tumor model In vitro   Breast cancer
Topoisomerase inhibition assay  In vitro   Cancer
Anti-proliferation Assay in Primary cell lines  In vitro screening for the compounds anti-proliferation activity   Cancer
Apoptosis assay in primary cells/ tumors -To evaluate the anti-cancerous properties of a particular compound using apoptosis assay  In vitro/ In vivo   Cancer
DNMT, HDAC, HAT activity assays - Epigenetic assays for aging and anti-cancer research. Assays being used to evaluate the epigenetic targets of novel compounds in mammalian cell lines. The major epigenetic targets are DNMTs, HATs and HDACs.  In vitro   Cancer, Aging 
Telomerase assay  In vitro   Cancer, Aging 
Chromatin Immuno Precipitation (ChIP) assay- For DNA-protein interaction studies  In vitro   Cancer / Male Reproduction/Neurobiology 
Comet Assay -For DNA damage  In vitro   Cancer, Male reproduction 
Transcription assay  In vitro   Cancer, Male reproduction  
Clonogenic assay  In vitro   Cancer
Proteasome inhibition assay –For screening of proteasome inhibitors  In vitro   Cancer, Male reproduction
Mammalian two hybrid assay- Cellular function  In vitro   Cancer, Male reproduction
Flowcytometric TUNEL assay-DNA damage, apoptosis  In vitro   Cancer, Male reproduction
PMN Phagocytosis assay- For screening of Phagocytosis  In vitro   Cancer, Male reproduction
Flurometry based autophagy assay- For screening of Autophagy  In vitro   Cancer, Male reproduction
Luciferase reporter assay- For evaluation of Transcriptional activity  In vitro   Cancer / Male Reproduction/Neurobiology

* Minimum Quantity of the compound required, running cost of the assay and time required for the assay shall be communicated on request


CSIR-CDRI : Cancer Screening Information
A.Criteria to be met by the Standardized Extracts /Purified compounds sent for Screening
  1. We do not accept crude extracts for screening.
  2. The supplier should ensure adequate amount required for carrying out all screening tests and the appropriate mode of shipment of the standardized extracts/ purified compounds to us.
  3. Solubility of the compounds should either be in water, Ethanol or DMSO.
  4. Purity of the compounds must be ascertained (HPLC Method).
  5. The Molecular weight of Compounds should preferably be < 800.
  6. The Screening Data obtained will be supplied on a CD.
  7. Director CSIR-CDRI reserves the right to accept /decline screening of standardized extracts / purified compounds from any source.
B.List of Tests Carried out in the Screening
  • MTT Cell viability Assay
  • XTT Cell viability Assay.
  • CCK-8 Cell viability Assay.
  • Annexin/FITC Flowcytometric Assay.
  • PI/DAPI based Cell cycle Analysis.