CNS - CVS & Related Disorders

Researchers are invited to make use of the assays detailed below.

Model   Assay System   Disease  
ACE activity (kit based) IC50 determination
 
In vitro Primary screening model   CVS/hypertension  
Collagen induced platelet aggregation IC50 determination
 
In vitro assay   CVS/Anti-platelet activity  
ADP induced platelet aggregation IC50 determination
 
In vitro assay   CVS/Anti-platelet activity  
Arachidonic acid induced platelet aggregation IC50 determination
 
In vitro assay   CVS/Anti-platelet activity  
Thromboxane A2 agonist (U46619) induced platelet aggregation IC50 determination
 
In vitro assay   CVS/Anti-platelet activity  
Thrombin time IC50 determination
 
In vitro assay   CVS/Anti-coagulant activity  
Prothrombin time IC50 determination
 
In vitro assay   CVS/Anti-coagulant activity  
Activated partial thromboplastin time IC50 determination
 
In vitro assay   CVS/Anti-coagulant activity  
Inhibition of adipogenesis
 
In vitro cell based assay   CVS/Anti-dyslipidemic  
Dexamethosone induced insulin resistance model in 3T3 L1 adipocyte-
 
In vitro cell based assay   CVS/Anti-dyslipidemic  
Chronic insulin model in 3T3 L1 adipocyte
 
In vitro cell based assay   CVS/Anti-dyslipidemic  
Endothelial cell assays
• Tubulogenesis Assay
• Aortic ring assay
• 3D fibrin assay

 
In vitro cell based assay   CVS/ Ischemia  
In vitro screening for Neuroprotective activity (PD)
 
In vitro cell based assay   CNS/ Neuroprotective  
Screening for selective novel ligands for GPCRs implicated in CNS diseases.
Following receptors are available for screening
• Dopamine family: D1-D5
• Serotonin family: 5-HT1A/B/D, ,5-HT2A/B/C; 5-HT6,
• Orphan GPCRs (GPR40, GPR120, GPR109A, TGR5)
• Muscarinic Receptors (M1-5)
• Histamine Receptor: H1-H3
• Opioid receptors: KOR DOR, MOR,

 
In vitro cell based assay   CNS/ GPCRs  
Vasoreactivity EC50 determination
 
In vitro Acetylcholine induced Vasorelaxation/ Phenylephrine induced vasoconstriction activity   CVS/Vasorelaxant/ vasoconstriction activity  
Anti-inflammatory activity • COX-1/COX2 (kit based) • TNFα Inhibition (kit based)
 
In vitro cell based assay   Other disorder Anti-inflammatory  

* Minimum Quantity of the compound required, running cost of the assay and time required for the assay shall be communicated on request






Model   Disease   Requirements  
Spontaneously hypertensive rats Continuous BP and HR recording by Telemetry in conscious SHR/SD rat
 
CVS/ hypertension   Provided on request  
Spontaneously hypertensive rats – For BP monitoring by non invasive blood pressure monitoring
 
CVS/ hypertension   Provided on request  
Monocrotaline induced pulmonary hypertension
 
CVS/ Pulmonary hypertension   Provided on request  
Collagen-adrenaline induced thrombosis (Single dose)
 
CVS/Anti-thrombotic   Provided on request  
Bleeding Time (Single dose)
 
CVS/Anti-thrombotic   Provided on request  
FeCl3 induced Thrombosis (Single dose)
 
CVS/Anti-thrombotic   Provided on request  
Arterio-Venous Shunt Thrombosis (Single dose)
 
CVS/Anti-thrombotic   Provided on request  
Anti-ischemic potential in myocardial infarction model in rats; Single dose
 
CVS/ anti-ischemic   Provided on request  
Endothelial cell assays
• In vivo matrigel angiogenesis assay
• Ear Angiogenesis in mice
 
CVS/ Ischemia   Provided on request  
Animal models for learning and memory
• Active and Passive Avoidance test/Morris Water Maze test
• Inhibition of Acetylcholinesterase activity
 
CNS/Learning and memory   Provided on request  
Parkinson’s models (6-OHDA induced rat model of Parkinson’s disease)
• Assessment of behavioural deficits by neurobehavioral tests
• Open field activity
• Rotarod
 
Neurodegeneration   Provided on request  
Anti depressant activity evaluation in CUS Model
• Tail suspensionTest
• Forced swim test
 
CNS/ Anti-anxiety   Provided on request  
Hyperlipidemic guinea model
• LDL Cholestrol
• HDL Cholestrol
• Triglycerides
• Total Cholestrol
 
CVS/anti-dyslipidemic   Provided on request  
Atherothrombosis in dyslipidemic hamsters (3 months fed on HFD)
• FeCl3 induced thrombosis
• Plasma lipids
• Expression of inflammatory cytokines
• Coagulation cascade parameters
• Platelet adhesion
• Platelet aggregation
• Vasoreactivity
 
CVS/anti-dyslipidemic   Provided on request  
Anti-inflammatory activity. Carrageenan induced paw edema in rat/mice
 
Anti-inflammatory   Provided on request  

* Minimum Quantity of the compound required, running cost of the assay and time required for the assay shall be communicated on request