Hands-on Workshops
Practical, research-oriented workshops designed to provide participants with hands-on exposure to advanced experimental techniques, laboratory instrumentation, data acquisition and analysis.
Learn • Demonstrate • Analyse
Both workshops combine lectures, wet-lab demonstrations and practical data analysis to connect scientific concepts with experimental applications.
Flow Cytometry-Based Cell Assays for Evaluating Inflammation and Immune Functions
Workshop Objectives
- Understand the principles of inflammation, immune cell biology, and their relevance to disease and therapeutics.
- Learn the fundamentals of flow cytometry and its applications in immune phenotyping and the evaluation of immunomodulatory and anti-inflammatory activities of natural products.
- Develop practical skills in assay design, data acquisition, analysis, and interpretation for flow cytometry-based immune functional assays.
Phenomaster: Automatic Metabolic and Behavioural Phenotyping System
Workshop Objectives
- Understand the principles and applications of metabolic phenotyping in laboratory animals.
- Learn to assess metabolic and ambulatory parameters using the PhenoMaster system.
- Evaluate the effects of natural products on metabolic and functional outcomes in preclinical models.
- Gain hands-on experience in the acquisition, analysis, and interpretation of metabolic phenotyping data.
- Explore applications of comprehensive metabolic monitoring in drug discovery and translational research.
Workshop Modules
Participants will gain exposure to experimental workflows, instrumentation and data analysis.
Flow Cytometry
- Fundamentals of Inflammation and Immune Responses
- Flow Cytometry: Principles, Instrumentation and Experimental Design
- Instrument Setup, Controls, Compensation and Quality Assurance
Cell Assays
- Cell Viability and Cytotoxicity Assessment
- Immune Cell Phenotyping and Activation
- Oxidative Stress and Intracellular ROS Measurement
- Apoptosis and Cell Death Analysis
Flow Data Analysis
- Gating Strategy and Data Acquisition
- Quality Control
- Quantification of Positive Cell Populations
- Mean Fluorescence Intensity Analysis
- Immune Cell Population and Subset Analysis
PhenoMaster System
- PhenoMaster Hardware and Software Setup
- Experimental Design and Software Operation
- Data Analysis and Interpretation
- Troubleshooting
Metabolic Analysis
- Metabolic and Gas Exchange Analysis
- Behavioral and Locomotor Activity Analysis
- Physiological Parameter Analysis
- Food and Water Intake Analysis
Translational Applications
- Natural Product Research
- Preclinical Evaluation
- Metabolic and Functional Phenotyping
- Drug Discovery and Translational Research
Who can Register?
The workshops are intended for Ayush clinicians, scientists and researchers interested in natural products, inflammation, immune functions, metabolic phenotyping and translational research.
Interested in the Workshops?
Registration is required to participate. Due to limited seat availability, eligible applicants will be selected by the Organizing Committee and confirmation will be shared via email.