Hands-on Workshops
Practical, research-oriented workshops designed to provide participants with hands-on exposure to advanced experimental techniques, laboratory instrumentation, data acquisition and analysis.
Learn • Demonstrate • Analyse
Both workshops combine lectures, wet-lab demonstrations and practical data analysis to connect scientific concepts with experimental applications.
Flow Cytometry-Based Cell Assays for Evaluating Inflammation and Immune Functions
Workshop Objectives
- Understand the principles of inflammation, immune cell biology, and their relevance to disease and therapeutics.
- Learn the fundamentals of flow cytometry and its applications in immune phenotyping and the evaluation of immunomodulatory and anti-inflammatory activities of natural products.
- Develop practical skills in assay design, data acquisition, analysis, and interpretation for flow cytometry-based immune functional assays.
Phenomaster: Automatic Metabolic and Behavioural Phenotyping System
Workshop Objectives
- Understand the principles and applications of metabolic phenotyping in laboratory animals.
- Learn to assess metabolic and ambulatory parameters using the PhenoMaster system.
- Evaluate the effects of natural products on metabolic and functional outcomes in preclinical models.
- Gain hands-on experience in the acquisition, analysis, and interpretation of metabolic phenotyping data.
- Explore applications of comprehensive metabolic monitoring in drug discovery and translational research.
Workshop Modules
Participants will gain exposure to experimental workflows, instrumentation and data analysis.
Flow Cytometry
- Fundamentals of Inflammation and Immune Responses
- Flow Cytometry: Principles, Instrumentation and Experimental Design
- Instrument Setup, Controls, Compensation and Quality Assurance
Cell Assays
- Cell Viability and Cytotoxicity Assessment
- Immune Cell Phenotyping and Activation
- Oxidative Stress and Intracellular ROS Measurement
- Apoptosis and Cell Death Analysis
Flow Data Analysis
- Gating Strategy and Data Acquisition
- Quality Control
- Quantification of Positive Cell Populations
- Mean Fluorescence Intensity Analysis
- Immune Cell Population and Subset Analysis
PhenoMaster System
- PhenoMaster Hardware and Software Setup
- Experimental Design and Software Operation
- Data Analysis and Interpretation
- Troubleshooting
Metabolic Analysis
- Metabolic and Gas Exchange Analysis
- Behavioral and Locomotor Activity Analysis
- Physiological Parameter Analysis
- Food and Water Intake Analysis
Translational Applications
- Natural Product Research
- Preclinical Evaluation
- Metabolic and Functional Phenotyping
- Drug Discovery and Translational Research
Who can Register?
The workshops are intended for Ayush clinicians, scientists and researchers interested in natural products, inflammation, immune functions, metabolic phenotyping and translational research.