|
Model/Assay
|
Assay System
|
Disease area
|
|
Sander-Cramer assay for evaluation of spermicidal activity
|
In vitro
|
Contraception |
|
Assay for vaginal contraception in Rabbit
|
In vivo
|
Contraception |
|
Evaluation of Anti-proliferative activity against human BPH-stromal primary cells
|
In vitro
|
Benign Prostatic Hyperplasia
|
|
In vivo assay for anti-BPH activity in rat model
|
In vivo
|
Benign Prostatic Hyperplasia
|
|
Anti-spermatogenic activity in rats
|
In vivo
|
Contraception
|
|
Wound healing assay
|
In vitro
|
|
|
Migration and invasion assays
|
In vitro
|
Cancer
|
|
DMBA-induced mammary tumor model
|
In vivo
|
Breast cancer
|
| Topoisomerase inhibition assay |
In vitro
|
Cancer
|
|
Anti-proliferation Assay in Primary cell lines
|
In vitro screening for the compounds anti-proliferation activity
|
Cancer
|
|
Apoptosis assay in primary cells/ tumors
-To evaluate the anti-cancerous properties of a particular compound using apoptosis assay |
In vitro/ In vivo
|
Cancer
|
|
DNMT, HDAC, HAT activity assays
- Epigenetic assays for aging and anti-cancer research.
Assays being used to evaluate the epigenetic targets of novel compounds in mammalian cell lines. The major epigenetic targets are DNMTs, HATs and HDACs.
|
In vitro
|
|
|
Telomerase assay |
In vitro |
Cancer, Aging
|
|
Chromatin Immuno Precipitation (ChIP) assay- For DNA-protein interaction studies |
In vitro |
Cancer / Male Reproduction/Neurobiology |
|
Comet Assay
-For DNA damage |
In vitro |
Cancer, Male reproduction |
|
Transcription assay |
In vitro |
Cancer, Male reproduction |
|
Clonogenic assay |
In vitro |
Cancer |
| Proteasome inhibition assay –For screening of proteasome inhibitors
|
In vitro |
Cancer / Male Reproduction |
| Mammalian two hybrid assay- Cellular function |
In vitro |
Cancer / Male Reproduction |
| Flowcytometric TUNEL assay-DNA damage, apoptosis |
In vitro |
Cancer / Male Reproduction |
| PMN Phagocytosis assay- For screening of Phagocytosis |
In vitro |
Cancer / Male Reproduction |
| Flurometry based autophagy assay- For screening of Autophagy |
In vitro |
Cancer / Male Reproduction |
| Middle cerebral artery occlusion- For Screening of neuroprotective activity |
In vitro |
Neurobiology |
| Luciferase reporter assay- For evaluation of Transcriptional activity |
In vitro |
Cancer / Male Reproduction/Neurobiology |
| Neonatal SD rats for calvarial osteoblast cell isolation.
-Use for assessing alkaline phosphatase activity as a measure of osteoblast differentiation and alizarin staining for osteoblast mineralization |
In vitro |
Osteoporosis/ Bone diseases |
| Peak bone mass measurements- Rats administered with compound showing promising in vitro activity. Treatment for one month, autopsied and bones and serum used for evaluating bone formation and resorption parameters. |
In vitro |
Osteoporosis/ Bone diseases |
| Adult SD osteopenic rat model. -Rats ovariectomized and left for a period of three months to develop osteopenia. Following this, osteopenic rats administered compound for three months, autopsied and bones and serum used for evaluating bone formation and resorption parameters |
In vitro |
Osteoporosis/ Bone diseases |
| Adult Ovariectomized Balb/c mice model. Mice ovariectomized and administered compound for one month, autopsied and bones and serum used for evaluating bone formation and resorption parameters |
In vitro |
Osteoporosis/ Bone diseases |
| Bone marrow culture from adult SD rats-For osteogenic differentiation |
Ex vivo |
Osteoporosis/ Bone diseases |
| Bone marrow culture from female Balb/c mice-For osteoclast differentiation |
Ex vivo |
Osteoporosis/ Bone diseases |
* Minimum Quantity of the compound required, running cost of the assay and time required for the assay shall be communicated on request