Focus on Malaria, Leishmania and Filaria

  • With prevalence in more than a 100 countries and more than 4 billion people worldwide at combined risk, diseases caused by these three parasites represent a major biomedical challenge. Researchers at the institute address issues pertaining to design and development of novel drug molecules as well as optimization and preclinical development of lead molecules and combination therapy regimens, besides investigation of novel drug delivery systems. A significant basic research component of the programme focuses on identification and characterization of novel drug targets, structure and molecular modelling for drug design, understanding mechanisms of drug action and drug resistance, investigation of aspects of parasite biology and host-parasite interaction, immunoprophylaxis and immunodiagnosis. The contribution of host genetic factors in malaria susceptibility in Indian populations is also of interest.


    Drug Discovery & Development
  • image01 Synthesis of compounds for anti-parasitic activity based on identified leads for rational drug design as well as isolation from natural products.

Bioevaluation against internationally accepted in vitro and in vivo experimental models as below:
    Exploration of Novel Drug Targets
  • image01 Characterization of unique biochemical pathways and enzymes for identification and validation of putative drug targets in Plasmodium, Leishmania and Brugia malayi.

  • image01 Elucidation of mechanism of drug resistance and development of targets for rational designing of new antileishmanials and antifilarials.

    Molecular and Cell Biology of Parasites
  • image01 Delineation of the mechanism of DNA replication and translation of the P. falciparum apicoplast genome.

  • image01 Molecular characterization of proteins involved in the SUF pathway of [Fe-S] biogenesis in the apicoplast.

  • image01 Generation of regular or conditional mutants in Plasmodium berghei for the functional characterization of Plasmodium proteins

  • image01 Addressing the role of DNA quadruplexes in Plasmodium gene regulation.

  • image01 Investigating mechanism of drug action of promising anti-leishmanial agents

  • image01 Actin network proteins and their function in Leishmania.

  • image01 Characterization of chaperonins in Leishmania and their interacting partners.

  • image01 Molecular and biochemical characterization of MAP Kinase1 in Leishmania, role in resistance and posttranslation modification of proteins.

  • image01 Molecular characterization of Wolbachia transcription elongation factor (Wol GreA) and translation initiation factor 1 (Wol Tl IF-1).

  • image01 Structural modeling and inhibitor identification using in silico and rational approaches.

  • image01 Investigating mechanism of drug action of promising anti-leishmanial agents
    Immunobiology
  • image01 Wolbachia - Immunological characterization of recombinant Wolbachia surface protein (WSP).

  • image01 Elucidating the immune evasion strategies adopted by the filarial parasite B. malayi at the earliest host-parasite interface.

  • image01 Identification and characterization of genes involved in the pathogenesis of Tropical Pulmonary Eosinophilia (TPE), a rare but fatal manifestation of Filariasis.

  • image01 Recombinant expression of cytokine homologues of B. malayi and immunological characterization of recombinant Wolbachia surface protein (WSP).

    Achievements


  • image01 Blood schizontocidal antimalarial E-Mal (licenced to Themis India Ltd. since 1997) is a fast acting, semi-synthetic artemisinin derivative effective against drug resistant and cerebral malaria cases. Post marketing surveillance shows quick and sustained recovery of comatose cerebral malaria cases and prevention of high mortality.

  • image01 Elubaquine an anti-relapse antimalarial was licenced to Nicholas Piramal India Ltd. in 2000 for treatment of relapses in P.vivax patients. Elubaquine an analog of primaquine produces 3 to 4 times lower methemoglobin toxicity thus providing an improved therapeutic index over primaquine



  • image01 Compound CDRI 97/78, a fully synthetic 1,2,4 trioxane derivative has been identified for development as a viable alternative to artemisinine derivatives for use against drug resistant P. falciparum and cerebral malaria cases. The compound has successfully completed preclinical efficacy and regulatory tests and Phase I clinical trials are in progress.

  • image01 Another antimalarial trioxane compound CDRI 99/411 with increased half life to allow for extended exposure after oral dosage has also completed preclinical efficacy and regulatory studies.
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  • image01 Clinical development of both the above compounds is being pursued under Licensing agreements with IPCA Pharmaceuticals Ltd., Mumbai.

  • image01 Oral formulations of arteether and fansidar as a synergistic combination for the treatment of multi drug resistant malaria has been developed.
    Basic Research


  • image01 An experimental arteether resistant rodent malaria model was established as a tool to study mechanism of resistance. Reporter gene based in vitro screening assays employing GFP and luciferase-transfected L. donovani parasites established.

  • image01 C57BL6 mice infected with P. berghei ANKA strain has been established as a cerebral malaria model.



  • image01 Interaction of antibiotics with translation factors and ribosomes of the apicoplast and mitochondrion of Plasmodium was investigated.

  • image01 Experimental evidence was provided for the existence of the SUF pathway of [Fe-S] cluster biogenesis in the apicoplastand the mobilization of sulphur from L-cysteine by the nuclear-encoded SufS (in conjunction with SufE) was demonstrated.

  • image01 B. malayi thymidylate kinase, trehalose 6-phosphate phosphatase, Wolbachia NAD+-dependent DNA ligase, UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and rsmD-like rRNA methyltransferase were characterized as putative drug targets against filaria.

  • image01 Leishmania drug targets such as pteridine reductase, trypanothionereductase, dipetidyl carboxypeptidase and -adenosyl-methionine decarboxylase, S-adenosyl methionine decarboxylase-like protein and Rab GTPases.

  • image01 Biochemical and molecular mechanism of drug resistance studied in Leishmania

  • image01 Wolbachia Transcription elongation factor ‘GreA’ and its two domains (NTD and CTD) were cloned and characterized and their structure is under elucidation. GreA interacts with α2ββ’σ Subunits of RNA Polymerase through dimeric CTD (as shown below in figure).


  • Three dimensional structure of BmL3-Helicase

  • image01 Translation initiation factor-1 of Wolbachia (Wol Tl IF-1) was cloned and characterized, its mutant was constructed and also characterized.

  • image01 Molecular cloning and characterization of Brugia malayi novel gene UDP galactopyranose mutase.

  • image01 Molecular cloning and characterization of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) of Wolbachia endosymbiont.



  • image01 Mechanism of DNA replication and ori activation of the P. falciparum apicoplast was established.

  • image01 Investigation of apicoplast targeting and DNA condensation properties of PfHU established the protein as the major DNA organization protein of the apicoplast genome.

  • image01 Translation factors involved in peptide chain initiation, elongation, release and ribosome recycling in Plasmodium organelles were identified and their functional characterization revealed several properties different from their bacterial counterparts.

  • image01 The role of actin and actin-related proteins in Leishmania biology was established.The role of actin depolymerising factor, ADF/cofilin, in actin-dynamics and flagellar assembly in Leishmania was demonstrated

  • image01 Proteins involved in the polyamine biosynthesis pathway are being characterized and S-adenosyl-methionine decarboxylase has been shown to form complexes with S-adenosyl methionine Decarboxylase-Like protein, which is unique to kinetopplastida and also Spermidine Synthetase. Structural and functional characterization of these complexes are in progress.

  • image01 Crystal structure of the coiled coil domain of L. donovani Coronin, an actin-binding protein, has been elucidated and confirmed using Small Angle X-ray Scattering methods. The domain associates as an anti-parallel four helix bundle, novel to this class of proteins, with an inherent asymmetry.

  • image01 Gamma subunit of TCP1 of L.donovani was cloned and characterized. Recombinant protein forms homo-oligomeric complex and is able to fold protein in ATP dependent manner.





  • image01 Development of several recombinant Leishmania proteins, identified as Th1 stimulatory molecules through proteomic approach, as fusion proteins for their prophylactic as well as therapeutic potential.

  • image01 Identification and synthesis of antigenic T- cell epitopes of potential L. donovani Th1 stimulatory proteins for the development of synthetic and recombinant chimeric vaccine against Visceral Leishmaniasis.

  • image01 Recombinant B. malayi trehalose-6-phosphate phosphatase (Bm-TPP), B. malayi myosin (BmAF-Myo), B. malayi ATPase RNA Helicase and Wolbachia NAD+-dependent DNA Ligase (wBm-LigA) generated a mixed Th1/Th2 or a biased T helper cell response and produced noticeable protection against infective larval challenge in rodents.

  • image01 Recombinant Bm-TPP and BmAF-Myo as subunit vaccine provided superior protection in animal model when used as cocktail antigen and generated more robust immune response.

  • image01 B. malayi DNA/ protein heterologous prime boost vaccine was much superior in prophylactic efficacy over DNA/DNA or protein/ protein immunization.

  • image01 Drug Resistance Mechanism: In Leishmania, MAPK1 has been shown to be has a role in antimony resistance. MAPK1 of Leishmania donovani modulates antimony susceptibility by down regulating P-glycoprotein efflux pumps.



  • image01 Understanding the role of human genetic polymorphisms (in genes encoding immune regulatory and adhesion molecules) in determining susceptibility to severe falciparum malaria in India populations.

  • image01 Deciphering the basic mechanism of macrophage-Leishmania interaction with special emphasis on identification of negative regulatory proteins by which Leishmania turns off the various signaling cascades in phagocytic cells.

  • image01 Functional characterization of murine splenic dendritic cell subtypes in experimental visceral leishmaniasis.

  • image01 Wolbachia endosymbiont of B. malayi elicits Th-17 mediated proinflammatory immune response through surface protein (WSP) and regulatory T cells neutralization in mice helps in parasite clearance by enhancing Th17 mediated pro-inflammatory response.

    Recent Publications
  • K Kanchan, P Jha, SS Pati, S Mohanty, SK Mishra, SK Sharma, S Awasthi, V Venkatesh and S Habib (2015) Interferon-γ (IFNG) microsatellite repeat and single nucleotide polymorphism haplotypes of IFN-α receptor (IFNAR1) associated with enhanced malaria susceptibility in Indian populations. Infection, Genetics and Evolution29: 6-14.

  • Devi HK, Gupta A, Kumar A, Singh C, Saxena J, Srivastava K, Puri SK, Dwivedi AK, Habib S and Misra A (2015) Preferential Targeting of Human Erythrocytes Infected with the Malaria Parasite Plasmodium falciparum via Hexose Transporter Surface Proteins. International Journal of Pharmaceutics483(1-2):57-62.

  • Haider A, Allen SM, Jackson KE, Ralph SA, and Habib S (2015)Targeting and function of proteins mediating translation initiation in organelles of Plasmodium falciparum. Molecular Microbiology. 96(4):796-814.

  • Kanchan K, Pati SS, Mohanty S, Mishra SK, Sharma SK, Awasthi S, Indian Genome Variation Consortium, Venkatesh V, and Habib S (2015). Polymorphisms in host genes encoding NOSII, C-reactive protein, and adhesion molecules thrombospondin and E-selectin are risk factors for Plasmodium falciparum malaria in India. European Journal of Clinical Microbiology and Infectious Diseases DOI: 10.1007/s10096-015-2448-0.

  • Bhartiya D, Chandramouli B, Kumar N. Co-evolutionary analysis implies auxiliary functions of HSP110 in Plasmodium falciparum.Proteins. 2015 Aug; 83(8):1513-25.

  • Bhardwaj J, Siddiqui AJ, Goyal M, Prakash K, Soni A, Puri SK, Srivastava M (2015). Host immune response is severely compromised during lethal Plasmodium vinckei infection. Parasitol Res. 114(9):3445-57.

  • Gunjan S, Singh SK, Ahmad H, Dwivedi AK and Tripathi R (2015). In-vitro interaction of mefloquine and clarithromycin: highly synergistic against chloroquine resistant clone of Plasmodium falciparum. Life Sciences 136, 126–132. Doi: 10.1016/j.lfs.2015.06.027.

  • Mastan BS, Kumari A, Gupta D, Mishra S, Kumar KA. Gene disruption reveals a dispensable role for Plasmepsin VII in the Plasmodium berghei life cycle. Mol Biochem Parasitol. 2014; 195(1):10-13.

  • Kumar K, Ramakrishna G, Gunjan S, Shukla AK, Pasam VR, Balaramnavar VM, Sharma A, Jaiswal S, Lal J, Tripathi R, Anubhooti, Ramachandran R, and Tripathi RP (2014) Identification of Novel Phenyl Butenonyl C-Glycosides with Ureidyl and Sulfonamidyl Moieties as Antimalarial Agents. dx.doi.org/10.1021/ml500211c | ACS Med. Chem. Lett. Vol.5 pp 878-883.

  • M. Sharma, K. Chauhan, R. K. Srivastava, S. V. Singh, K. Srivastava, J. K. Saxena, S. K. Puri, P. M. S. Chauhan. Design and synthesis of new class of 4-aminoquinolinyl- and 9-anilinoacrydinyl schiff base hydrazones as potent antimalarial agents Chem. Biol. & Drug Des. 2014, 84(2), 175-181.

  • M. Sinha, V. Dola, P. Agarwal, K. Srivastava, W. Haq, S. K. Puri, S. B. Katti Antiplasmodial activity of new 4-Aminoquinoline derivatives against chloroquine resistant strain Bioorg. Med. Chem.2014, 22(14), 3573-86.

  • A. Mishra, H. Batchu, K. Srivastava, P. Singh, P. K Shukla, Sanjay Batra. Synthesis and evaluation of new diaryl ether and quinoline hybrids as potential antiplasmodial and antimicrobial agents. Bioorg. Med. Chem. Lett. 2014, 24(7), 1719-23.

  • M. Sinha, V. Dola, A. Soni, P. Agarwal, K. Srivastava, W. Haq, S. K. Puri, S. B. Katti Synthesis of chiral chloroquine and its analogues as antimalarial agents Bioorg. Med. Chem.2014, 22(14), 5950-60.

  • R. Sharma, A. K. Pandey, P. Verma, K. Srivastava, S. K. Puri, S. V. Singh, J. K. Saxena, P. M. S. Chauhan. 4-Aminoquinoline-Schiff base Hybrid: Rational Drug Design Approach for Overcoming Chloroquine Resistance in Malaria Chem. Biol. Interface 2014, 4(5), 268-280

  • R. Sharma, A. K. Pandey, P. Verma, K. Srivastava, S. K. Puri, S. V. Singh, J. K. Saxena, P. M. S. Chauhan. 4-Aminoquinoline-Schiff base Hybrid: Rational Drug Design Approach for Overcoming Chloroquine Resistance in Malaria Chem. Biol. Interface 2014, 4(5), 268-280

  • A. K. Pandey, R. Sharma, A. Singh, S. Shukla, K. Srivastava, S. K. Puri, B. Kumar, P.M.S. Chauhan. Synthesis of Biologically Active Pyridoimidazole /Imidazobenzothiazole Annulated Polyheterocycles Using Cyanuric Chloride in Water. Chem. Med. Comm. 2014, 4, 26757-26770

  • Tripathi R, Rizvi A, Pandey SK, Dwivedi H, Saxena JK (2013). Ketoconazole, a cytochrome P450 inhibitor can potentiate the antimalarial action of α/β arteether against MDR P. yoelii nigeriensis. ActaTropica 126, 150-155

  • Narender T,Venkateswarlu K, Papireddy SK, Khaliq T, Awakash S, Srivastava RK, Srivastava K, Puri SK, Raju KSR, Wahajuddin, Sijwali PS, Kumar V, and Siddiqi MI (2013). Synthesis and Insight into the Structure – Activity Relationships of Chalcones as Antimalarial Agents, Journal of Medicinal Chemistry, 56, 31-45


  • Rahul Shukla, Jyoti Gupta, Prashant Shukla, Pankaj Dwivedi, Priyanka Tripathi, Shailja M. Bhattacharya and Prabhat R. Mishra (2015).Chitosan coated alginate microparticles for the oral delivery of antifilarial drugs and combinations for intervention in Brugia malayi induced lymphatic filariasis. RSC Advances; 5, 69047-69056; Doi:10.1039/C5RA06982C.

  • Nidhi Shrivastava, Jeetendra K. Nag, Jyoti Pandey, Rama Pati Tripathi, Priyanka Shah, Mohammad Imran Siddiqi and Shailja Misra-Bhattacharya (2015). Homology modeling of NAD+-dependent DNA ligase of Brugia malayi Wolbachia and its drug target potential using dispiro-cycloalkanones. Antimicrobial Agents and Chemotherapy; DOI: 10.1128/AAC.03449-14.

  • Manisha Pathak, Meenakshi Verma, Mrigank Srivastava and Shailja Misra-Bhattacharya (2015). Wolbachia endosymbiont of Brugia malayi elicits Th-17 mediated proinflammatory immune response through surface protein (WSP). Immunology, 144(2) 231-244. doi: 10.1111/imm.12364. PMID:25059495.

  • Mohd Shahab, Meenakshi Verma, Manisha Pathak, Kalyan Mitra and Shailja Misra-Bhattacharya (2014). Cloning, Expression and Characterization of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Wolbachia Endosymbiont of Human Lymphatic Filarial Parasite Brugia malayi. PLoS One.;9(6):e99884. doi: 10.1371/journal.pone.0099884. eCollection 2014.

  • Jeetendra Kumar Nag, Nidhi Shrivastava, Manish Tiwari, Chhedi Lal Gupta, Preeti Bajpai, Dhanvantri Chahar, Shailja Misra-Bhattacharya (2014). Wolbachia translation initiation factor-1 is copiously expressed by the adult, microfilariae and infective larvae of Brugia malayi and competitively inhibited by tetracycline. Acta Tropica, 138, 51-59, doi: 10.1016/j.actatropica.2014.04.033. Epub 2014 Jun 11

  • Chandradev Pati Tripathi, Reema Gupta, Pramod Kumar Kushwaha, Chitra Mandal, Shailja Misra-Bhattacharya and Anuradha Dube (2014). Comparative Immunoprophylactic efficacy of Withania somnifera chemotypes NMITLI - 101R, NMITLI - 118R and Withaferin A against Leishmania donovani infection in golden hamsters. Parasite Immunology, 253–265. doi: 10.1111/pim.12112.PMID: 24830833

  • Susheela Kushwaha, Prashant Kumar Singh, Ajay Kumar Rana and Shailja Misra-Bhattacharya (2013). Immunization of Mastomys coucha with Brugia malayi recombinant trehalose-6-phosphate phosphatase results in significant protection against homologous challenge infection. PLOS ONE,8(8):e72585. doi: 10.1371/journal.pone. 0072585.

  • Ajay Kumar Rana, Sharat Chandra, Mohammad Imran Siddiqi, Shailja Misra-Bhattacharya (2013). Molecular characterization of an rsmD-like rRNA methyltransferase from Wolbachia endosymbiont of Brugia malayi and antifilarial activity of specific inhibitors of the enzyme. Antimicrobial Agents and Chemotherapy, 57 (8) 3843–3856

  • K.V. Sashidhara, S. Rao Avula, P.K. Doharey, L. R. Singh, V.M. Balaramnavar, J. Gupta, S. Misra-Bhattacharya, S. Rathaur, A.K. Saxena and J.K. Saxena (2015). Designing, synthesis of selective and high-affinity chalconebenzothiazole hybrids as Brugia malayi thymidylate kinase inhibitors: in vitro validation and docking studies. European J Med Chem, 103, 418-4281.

  • Pathak M, Verma M, Srivastava M, Misra-Bhattacharya S. (2015).Wolbachia endosymbiont of Brugia malayi elicits a T helper type 17-mediated pro-inflammatory immune response through Wolbachia surface protein. Immunology 144(2):231-44

  • Charan M, Singh N, Kumar B, Srivastava K, Siddiqi MI, and Habib S (2014) Sulphur mobilisation for [Fe-S] cluster assembly by the essential SUF pathway in the Plasmodium falciparumapicoplast and its inhibition. Antimicrobial Agents and Chemotherapy58(6): 3389-3398.

  • Gupta A, Shah P, Haider A, Gupta A, Siddiqi MI, Ralph SA and Habib S (2014) Reduced ribosomes of the apicoplast and mitochondrion of Plasmodium spp. and predicted interactions with antibiotics. Open Biology 4(5), 140045. doi 10.1098/rsob.140045

  • M. Sharma, K. Chauhan, R. K. Srivastava, S. V. Singh, K. Srivastava, J. K. Saxena, S. K. Puri, P. M. S. Chauhan. Design and synthesis of new class of 4-aminoquinolinyl- and 9-anilinoacrydinyl schiff base hydrazones as potent antimalarial agents Chem. Biol. & Drug Des. 2014, 84(2), 175-181.

  • M. Sinha, V. Dola, P. Agarwal, K. Srivastava, W. Haq, S. K. Puri, S. B. Katti Antiplasmodial activity of new 4-Aminoquinoline derivatives against chloroquine resistant strain Bioorg. Med. Chem.2014, 22(14), 3573-86

  • A. Mishra, H. Batchu, K. Srivastava, P. Singh, P. K Shukla, Sanjay Batra. Synthesis and evaluation of new diaryl ether and quinoline hybrids as potential antiplasmodial and antimicrobial agents. Bioorg. Med. Chem. Lett. 2014, 24(7), 1719-23

  • M. Sinha, V. Dola, A. Soni, P. Agarwal, K. Srivastava, W. Haq, S. K. Puri, S. B. Katti Synthesis of chiral chloroquine and its analogues as antimalarial agents Bioorg. Med. Chem.2014, 22(14), 5950-60

  • R. Sharma, A. K. Pandey, P. Verma, K. Srivastava, S. K. Puri, S. V. Singh, J. K. Saxena, P. M. S. Chauhan. 4-Aminoquinoline-Schiff base Hybrid: Rational Drug Design Approach for Overcoming Chloroquine Resistance in Malaria Chem. Biol. Interface 2014, 4(5), 268-280

  • A. K. Pandey, R. Sharma, A. Singh, S. Shukla, K. Srivastava, S. K. Puri, B. Kumar, P.M.S. Chauhan. Synthesis of Biologically Active Pyridoimidazole /Imidazobenzothiazole Annulated Polyheterocycles Using Cyanuric Chloride in Water. Chem. Med. Comm. 2014, 4, 26757-26770

  • Gupta A, Mir SS, Jackson KE, Lim EE, Shah P, Sinha A, Siddiqi MI, Ralph SA and Habib S (2013) Recycling factors for ribosome disassembly in the apicoplast and mitochondrion of Plasmodium falciparum. Molecular Microbiology 88(5): 891-905.

  • Tanveer A, Allen SM, Jackson KE, Charan M, Ralph SA, and Habib S (2013) An FtsH protease is recruited to the mitochondrion of Plasmodium falciparum. PLoS ONE. vol. 8, issue 9, e74408.

  • Gupta A, Mir SS, Saqib U, Biswas S, Vaishya S, Srivastava K, Siddiqi MI, and Habib S (2013)The effect of fusidic acid on Plasmodium falciparum elongation factor G (EF-G). Molecular & Biochemical Parasitology 192: 39– 48.

  • Identification and characterization of genes involved in the pathogenesis of Tropical Pulmonary Eosinophilia (TPE), a rare but fatal manifestation of Filariasis.


  • Mansi Garg and Neena Goyal (2015) MAPK1 of Leishmania donovani Modulates Antimony Susceptibility by Downregulating P-Glycoprotein Efflux Pumps. Antimicrob Agents Chemother. 59:3853-63.

  • Shikha S. Chauhan, Shashi Pandey, Rahul Shivahare, Karthik Ramalingam, Shagun Krishna, Preeti Vishwakarma, M. I. Siddiqi, Suman Gupta, Neena Goyal and Prem M. S. Chauhan (2014). Novel b-carboline–quinazolinone hybrid as an inhibitor of Leishmania donovani trypanothione reductase: synthesis, molecular docking and bioevaluation Med. Chem. Comm c4md00298a

  • Goyal N. (2013 ) Novel approaches for the identification of inhibitors of leishmanial dipeptidylcarboxypeptidase.Expert Opin Drug Discov. 8(9):1127-34

  • Smita Rai, Bhaskar, Sudhir K Goel, Upendra nath Dwivedi, Shyam sundar, Neena Goyal (2013). Role of efflux pumps and intracellular thiols in natural antimony resistant isolates of leishmania donovani. Plos One 8(9): e74862. doi:10.1371/journal.pone.0074862

  • Gupta CM, Thiyagarajan S, Sahasrabuddhe AA. (2015) Unconventional actins and actin-binding proteins in human protozoan parasites. Int J Parasitol. 45:435-47.

  • Singh K, Veluru NK, Trivedi V, Gupta CM, Sahasrabuddhe AA. (2014) An actin-like protein is involved in regulation of mitochondrial and flagellar functions as well as in intramacrophage survival of Leishmania donovani. Mol Microbiol. 91:562-78.

  • Baharia RK, Tandon R, Sharma T, Suthar MK, Das S, Siddiqi MI, Saxena JK, Sundar S, Dube A. (2015) Recombinant NAD-dependent SIR-2 protein of Leishmania donovani: immunobiochemical characterization as a potential vaccine against visceral leishmaniasis. PLoS Negl Trop Dis. 9:e0003557.

  • Shukla VK, Kabra A, Maheshwari D, Yadav R, Jain A, Tripathi S, Ono S, Kumar D, Arora A. (2015) Solution structures and dynamics of ADF/cofilins UNC-60A and UNC-60B from Caenorhabditis elegans. Biochem J. 465:63-78.

  • Shukla VK, Kabra A, Yadav R, Ono S, Kumar D, Arora A. (2015) NMR assignments of actin depolymerizing factor (ADF) like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans. Biomol NMR Assign. 9:261-5.

  • Jaiswal AK, Khare P, Joshi S, Kushawaha PK, Sundar S, Dube A. (2014)Th1 stimulatory proteins of Leishmania donovani: comparative cellular and protective responses of rTriose phosphate isomerase, rProtein disulfide isomerase and rElongation factor-2 in combination with rHSP70 against visceral leishmaniasis. PLoS One. 9:e108556.

  • Gupta R, Kumar V, Kushawaha PK, Tripathi CP, Joshi S, Sahasrabuddhe AA, Mitra K, Sundar S, Siddiqi MI, Dube A. (2014) Characterization of glycolytic enzymes--rAldolase and rEnolase of Leishmania donovani, identified as Th1 stimulatory proteins, for their immunogenicity and immunoprophylactic efficacies against experimental visceral leishmaniasis. PLoS One. 9:e86073.

  • Zahir AA, Chauhan IS, Bagavan A, Kamaraj C, Elango G, Shankar J, Arjaria N, Roopan SM, Rahuman AA, Singh N. (2015) Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G0/G1 Arrest followed by Necrotic Cell Death in Leishmania donovani. Antimicrob Agents Chemother. 59(8):4782-99

  • Neeloo Singh, Mitali Chatterjee and Shyam Sundar (2014) The overexpression of genes of thiol metabolisim contribute to drug resistance in clinical isolates of visceral leishmaniasis (kala azar) in India. Parasites and Vectors 7: 596

  • Singh S.P., Agnihotri P. and Pratap J.V. (2013) Characterization of a novel putative S-adenosylmethionine decarboxylase-like protein from Leishmania donovani. PLOS ONE (8), 6: 10.1371/0065912.

  • Mishra AK, Agnihotri P, Srivastava VK, Pratap JV (2015) Novel protein-protein interaction between spermidine synthase and S-adenosylmethionine decarboxylase from Leishmania donovani. Biochem Biophys Res Commun. 2015 Jan 9;456(2):637-42.

  • Shivahare R, Vishwakarma P, Parmar N, Yadav PK, Haq W, Srivastava M, Gupta S, Kar S. (2014). Combination of liposomal CpGoligodeoxynucleotide 2006 and miltefosine induces strong cell-mediated immunity during experimental visceral leishmaniasis.PLoS One. 14;9(4):e94596

  • Shivahare R, Korthikunta V, Chandasana H, Suthar MK, Agnihotri P, Vishwakarma P, Chaitanya TK, Kancharla P, Khaliq T, Gupta S, Bhatta RS, Pratap JV, Saxena JK, Gupta S, and Narender T (2014). Synthesis, structure–activity relationships, and biological Studies of Chromenochalcones as potential antileishmanial agents J. Med.Chem , 57, 3342-3357

Team Members
 
Dr. Saman Habib Dr. Saman Habib Dr. Neena Goyal
Dr. Neena Goyal Dr. Satish Mishra Dr. Neeloo Singh
Dr. Sanjay Batra Dr. Renu Tripathi Dr. Amogh A. Sahasrabuddhe
  Dr. Kumkum Srivastava Dr. Susanta Kar
  Dr. Niti Kumar Dr. Kalyan Mitra
     
Dr. Mrigank Srivastava Dr. MI Siddiqi Dr. PMS Chauhan
  Dr. R. Ravishankar Dr. RP Tripathi
  Dr. Ashish Arora Dr. Sanjay Batra
  Dr. J.V. Pratap Dr. PP Yadav
    Dr. K Mohanan
    Dr. Namrata Rastogi
 
Dr. T. Narender Dr. AK Dwivedi  
Dr Rakesh Maurya Dr. Amit Misra  
Dr. KV Sashidhara Dr. Prabhat Mishra  
  Dr. Wahajuddin  
  Dr. Jawahar Lal  
  Dr. Ravi Bhatta