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Research Interests

Adipocyte Biology Lab

Basic sciences
Our lab have developed physiologically relevant in-vitro model of adipocyte insulin resistance using murine pre-adipocyte cell line as well as stem cell derived human adipocyte. Similarly in-vivo model system of physiological chronic hyperinsulinemia is developed exploiting continuous infusion of exogenous insulin by mini-osmotic pump/implants. Broad interests of our lab are in following domains.

image01 Mechanisms of adipocyte insulin resistance.
image01 Stem cells to Adipose Differentiation/ Trans-differentiation studies.
image01 Systems Biology approach to study adipocytes insulin resistance.
image01 Adipose Immuno-metabolic alterations.
image01 Adipocyte Secretome and Endocrine functions.
image01 Adipocyte miRNA profiling and functional evaluations.
image01 Physiological lipids and their importance in overall energy homeostasis.

We use various signaling, differential gene and protein expression, quantitative gene expression studies, adipokine antibody arrays, Protein over-expression and suppression approaches using siRNA /Lentiviral particles etc. Adipocytes engaged in studies are derived either from cell line (3T3-L1, C3H10T1/2) or characterized human primary mesenchymal stem cells.

Drug Discovery and development

Our lab contributes mainly in following areas of drug discovery and development.

image01 Anti-adipogenic and anti-dyslipidemic drug discovery: Our lab has reported observed inter-relationship of anti-adipogenic and anti-dyslipidemic activities. We have also developed Common Feature Pharmacophore model of anti-adipgenic activity (CFPMA). This allowed us to screen our in house natural product library to identify novel natural molecules possessing both activities.
image01 Anti-obesity activity profiling: We use high fat diet induced obesity model of C57BL/6 mice. Compounds possessing anti-adipogenic activity are profiled for this activity too.
image01 Anti-diabetic activity profiling: We use various model systems to study anti-diabetic activity profiling of compounds. Low dose Streptozotocin and high fructose rat model, STZ induced diabetic rat model, Glucocorticoid induced diabetic mice model, Leptin receptor deficient genetic model of diabetes i.e. db/db mice and HFD induced diabetic mice model.